PXD060106

PXD060106 is an original dataset announced via ProteomeXchange.

Title	APEX2-based quantitative proteomic study of LAT and CD3z in Jurkat T cells
Description	TCR binding to its ligands induces a cascade of signaling starting by the phosphorylation of immunoreceptor tyrosine-based activation motif (ITAMs) present in the TCR-associated CD3 complex. This is followed by the phosphorylation of proteins including the adaptor LAT, which once phosphorylated interacts with multiple proteins allowing signal diversification and amplification downstream of the TCR. We take advantage of peroxidase-catalyzed proximity labeling combined with quantitative mass spectrometry to track the formation of CD3z and LAT interactome dynamics in Jurkat cells after TCR stimulation. We find more than 1 000 high confidence proteins for each bait and provide a quantitative molecular map of proteins enriched or reduced from the vicinity of CD3z and LAT, after stimulation. We detail and compare the recruitment kinetics of signaling proteins to CD3z and LAT and identify previously uncharacterized mediators of T cell activation. We show that the kinase MARK2, which is in the proximity of LAT and CD3z at resting state and lost upon activation, is a negative regulator of cytokine production by T cells. This study provides a resource for uncovering the complex signaling networks that regulate TCR activation and highlights new players of the TCR-induced signaling cascade.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_10:35:31.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Masson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-23 03:21:25	ID requested
⏵ 1	2025-09-02 10:35:32	announced

Dataset with its publication pending

submitter keyword: TCR signaling, T lymphocyte,proximity ligation assay

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliation	Institut Curie - Centre de Recherche
contact email	vanessa.masson@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD060106

PRIDE project URI

• PRIDE
  1. PXD060106
     1. Label: PRIDE project
     2. Name: APEX2-based quantitative proteomic study of LAT and CD3z in Jurkat T cells