Recent studies on the contribution of satellite cells (SC) to Duchenne muscular dystrophy (DMD) documented altered division capacities and impaired regeneration potential of SC in muscle tissue of DMD patients and animal models. Hence, it is crucial to unravel if SC-intrinsic effects trigger these deficiencies at pre-contractile stages of myogenesis rather than resulting consequently from the pathologic environment. Addressing this, we isolated SC from muscle biopsies of a porcine DMD model for cultivation and characterization.