HEK293T cells were transfected with GFP-poly-G-TurboID or GFP-TurboID as a control. Following biotin labelling, the cells were lysed in RIPA buffer to separate the soluble and insoluble fractions. The insoluble proteins in the pellet containing poly-G aggregates were further lysed in the lysis buffer containing 8 M urea to ensure effective solubilization