In this study, 4 PCOS serum samples and 4 healthly controls were utilized for proteomic analysis. The UltiMate 3000 (Thermo Fisher Scientific, MA, USA) liquid chromatography system was connected to the timsTOF Pro 2, an ion-mobility spectrometry quadrupole time of flight mass spectrometer (Bruker Daltonik, Bremen, Germany). For comparative analysis of PCOS and healthy controls, FC> 1.2 and p-value <0.05 were selected as differential protein screening criteria. Deferentially expression proteins were analysised using gene ontology (GO) terms with Blast2GO software (http://www.blast2go.com/b2ghome).GO annotation results were plotted using R scripts. and matched against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database by the KEGG Automatic Annotation Server (KAAS, https://www.genome.jp/tools/kaas/). P < 0.05 in Fisher’s exact test was considered significant. The STRING-DB protein interaction network was built using the STRING database (http://string-db.org/).