This project focuses on the proteomic analysis of biofilm formation in Aeromonas hydrophila LP-2. By comparing the wild-type strain and the orf-2341 deletion mutant, we aim to identify biofilm-associated proteins and explore the role of orf-2341 in outer membrane protein assembly and compensatory pathways. The study utilizes advanced proteomics techniques, including LC-MS/MS, to uncover differential protein expression patterns.