This study utilizes a label-free quantitative proteomics approach to analyze protein digests using mass spectrometry. Unlike isotope labeling methods, this technique avoids the use of expensive internal standards. The principle is based on the extraction of ion chromatograms (XICs) from peptide precursor ions, allowing identification of peptides and proteins in samples followed by quantitative analysis. This method not only enhances the speed of quantification but also improves the accuracy of the results.