To elucidate the function of the histidine kinase ONR73_07150 in Aeromonas veronii, we employed label-free quantitative proteomics to compare the total proteomes of wild-type and ONR73_07150 knockout strains. This approach identified a total of 2614 proteins, of which 52 were differentially expressed. Subsequently, we performed functional annotation of these differentially expressed proteins using Gene Ontology analysis.