PXD059551

PXD059551 is an original dataset announced via ProteomeXchange.

Title	• Optimal Functioning of the Lipopolysaccharide Transport Bridge Depends on a Ternary Complex Between the Lipocalin YedD and the LptDE Translocon
Description	The outer membrane is an efficient permeability barrier that protects Gram-negative bacteria against external assaults including many antibiotics. The unique permeability features of the outer membrane are due to the presence of lipopolysaccharide (LPS) molecules in its outer leaflet. LPS localization relies on the essential Lpt pathway, which forms a protein bridge that conveys LPS molecules from the inner membrane to the outer membrane. On the receiving side are two outer membrane components, LptD and LptE, which form the translocon that inserts LPS molecules in the outer leaflet. Here, we identify the lipocalin YedD as a novel component of the outer membrane LPS translocon. Structural analysis of the YedD-LptDE complex using cryo-electron microscopy reveals that YedD binds LptD to a region between the -barrel and the periplasmic -taco domain, critical for LptD function. The YedD-LptDE complex is functionally relevant: under conditions where the connectivity of the -taco and Lpt bridge is compromised, the absence of YedD leads to decreased cell viability and accumulation of LPS in the inner membrane. Our findings establish YedD as a novel Lpt component required for optimal LPS transport
HostingRepository	PRIDE
AnnounceDate	2025-07-09
AnnouncementXML	Submission_2025-07-09_06:44:22.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-08 06:28:47	ID requested
⏵ 1	2025-07-09 06:44:23	announced

10.1016/j.celrep.2025.115446;

Gennaris A, Nguyen VS, Thouvenel L, Csoma N, Vertommen D, Iorga BI, Remaut H, Collet JF, Optimal functioning of the Lpt bridge depends on a ternary complex between the lipocalin YedD and the LptDE translocon. Cell Rep, 44(4):115446(2025) [pubmed]

submitter keyword: LPS,bacteria, lipocalin, outer membrane, translocon

Jean-François Collet
contact affiliation	de Duve Institute, Université catholique de Louvain (UCLouvain), Avenue Hippocrate 75, 1200 Brussels, Belgium
contact email	jfcollet@uclouvain.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059551
     1. Label: PRIDE project
     2. Name: • Optimal Functioning of the Lipopolysaccharide Transport Bridge Depends on a Ternary Complex Between the Lipocalin YedD and the LptDE Translocon