This study investigates the proteomic alterations in pancreatic cancer cells resulting from Serpinb9 knockdown (KD) compared to normal control (NC). Two experimental groups, each consisting of three biological replicates, were analyzed to identify key proteins and pathways associated with Serpinb9's role in pancreatic cancer. Protein identification and quantification were performed using high-resolution mass spectrometry to provide insights into molecular mechanisms underlying its biological function