Tetraspanin7 (Tspan7) is involved in the regulation of body weight, but the detailed mechanisms remain unclear. Here, to reveal the mechanisms, human adipose-derived stem cells were transduced with sh-Tspan7, control-sh, Tspan7-Flag, and Myc-Tspan7 using lentivirus. Following the transduction, adipogenic differentiation was induced and the differentiated adipocytes were characterized by LC-MS/MS.