PXD059025

PXD059025 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics of Cochlear Tissues: Bilateral Comparisons in Guinea Pigs and Rats
Description	The cochlea, a sensitive biosensor, detects sound waves and converts them into electrical signals the brain recognizes as sound. Damage to cochlear hair cells can release proteins, triggering biological responses that may impair hearing. Mass spectrometry-based proteomics offers insights into protein expression changes in cochlear tissues, improving our understanding of inner ear diseases. In this study, we performed a comprehensive proteomics analysis of whole cochlear tissue extracted from healthy guinea pigs and rats. The study optimized protein extraction protocols and analyzed cochlear protein expression using three biological replicates for each animal model. This work optimized protein extraction protocols and analyzed cochlear protein expression using three biological replicates for each animal model. The results identified 1801 proteins in guinea pigs and 3867 proteins in rats, with a high overlap in cochlear protein expression between the left and right ears—93% in guinea pigs and 89% in rats. The findings validate the assumption that the cochlear tissues from both sides of the ears can be considered biologically equivalent. This experiment provides a comprehensive cochlear proteome for guinea pigs and rats, supporting future studies on inner ear disorders.
HostingRepository	PRIDE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-20_19:17:56.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Motahare Khorrami
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Cavia porcellus (Guinea pig); NCBI TaxID: 10141;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 20:20:37	ID requested
⏵ 1	2025-07-20 19:17:57	announced

Khorrami M, Haynes PA, Pastras C, Asadnia M, Quantitative Proteomics of Cochlear Tissues: Bilateral Comparisons in Guinea Pigs and Rats. Proteomics, 25(13):e13977(2025) [pubmed]

10.1002/pmic.13977;

submitter keyword: Mass-spectrometry, Cochlear tissue,Rat, Proteomics, Guinea pigs

Paul A Haynes
contact affiliation	Faculty of Science and Engineering, School of Natural Sciences, Macquarie University, Macquarie Park, Sydney 2109, NSW, Australia
contact email	paul.haynes@mq.edu.au
lab head
Motahare Khorrami
contact affiliation	Faculty of Science and Engineering, School of Engineering, Macquarie University, Sydney 2109, NSW, Australia
contact email	Motahare.khorrami@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059025

PRIDE project URI

• PRIDE
  1. PXD059025
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics of Cochlear Tissues: Bilateral Comparisons in Guinea Pigs and Rats