PXD058908

PXD058908 is an original dataset announced via ProteomeXchange.

Title	Analysis of proteins proximal to epsin 1 in A549 cells during entry of influenza A virus
Description	Influenza A viruses (IAVs) are respiratory pathogens that represent a global concern due to their ability to cause seasonal epidemics and sporadic pandemics in the human population. Thus, a comprehensive understanding of IAV biology is essential in order to design effective antivirals. It is well established that entry of most IAVs begins through binding to cell surface sialic acid, however the identity of receptors that mediate virus internalization remains unclear. Here, we performed TurboID-based proximity labelling centered on epsin 1, a protein required for clathrin-mediated endocytosis of IAV, to uncover internalization receptors. By performing affinity-based purification of biotinylated proteins coupled to mass spectrometry, we identified 34 proteins proximal to epsin 1 during IAV infection. Therein we found 11 high-confidence STRING interactors for epsin 1 and 3 putative pro-viral host factors for IAV infection, validating our approach.
HostingRepository	PRIDE
AnnounceDate	2025-05-08
AnnouncementXML	Submission_2025-05-08_07:56:35.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Milagros Sempere Borau
SpeciesList	scientific name: Influenza A virus (A/WSN/1933(H1N1)); NCBI TaxID: 382835; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-16 08:10:37	ID requested
⏵ 1	2025-05-08 07:56:35	announced

Dataset with its publication pending

submitter keyword: epsin 1,proximity labelling, virus entry, TurboID, influenza A virus

Silke Stertz
contact affiliation	Institute of Medical Virology, University of Zurich
contact email	stertz.silke@virology.uzh.ch
lab head
Milagros Sempere Borau
contact affiliation	Institute of Medical Virology, University of Zurich
contact email	sempereborau.milagros@virology.uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058908
     1. Label: PRIDE project
     2. Name: Analysis of proteins proximal to epsin 1 in A549 cells during entry of influenza A virus