PXD058907

PXD058907 is an original dataset announced via ProteomeXchange.

Title	Proteomics and bioinformatics guided discovery of microalgal multifunctional peptides for novel nutraceutical applications
Description	This study aimed to identify and characterize bioactive peptides derived from protein hydrolysates of Arthrospira platensis (APPH) and Tetraselmis chuii (TCPH) using an integrated peptidomics and bioinformatics approach. Proteins extracted from the microalgae were hydrolyzed using pepsin (EC 3.4.23.1) at various enzyme/substrate (E/S) ratios. APPH and TCPH, prepared at an E/S ratio of 1/10 (w/w), were analyzed using peptidomics through reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with tandem mass spectrometry (MS/MS). Using the UniProtKB database, a total of 265 unique peptides were identified, including 187 peptides from APPH and 78 peptides from TCPH. Subsequent in silico analysis of these peptides revealed favorable physicochemical properties, with a notable distribution of hydrophobic (APPH: 26; TCPH: 5), amphipathic (APPH: 70; TCPH: 16), and hydrophilic peptides (APPH: 59; TCPH: 17). Toxicity assessments confirmed that none of the peptides showed hemolytic or cytotoxic risks, except for one peptide identified in TCPH with potential cytotoxicity. Furthermore, bioactivity predictions demonstrated significant multifunctional properties (scores exceeding the 0.500 threshold), identifying peptides with antihypertensive (APPH: 2; TCPH: 1), anti-diabetic (APPH: 2), anti-inflammatory (APPH: 14; TCPH: 5) and antimicrobial (APPH: 7) activities. The current study thus establishes protein hydrolysates from A. platensis and T. chuii as promising sources of bioactive peptides suitable for nutraceutical applications. Our integrated analytical and computational strategy provides critical insights into peptide multifunctionality, supporting further research and development of microalgae-derived peptides.
HostingRepository	PRIDE
AnnounceDate	2025-07-09
AnnouncementXML	Submission_2025-07-09_05:22:08.452.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Deracinois
SpeciesList	scientific name: Arthrospira platensis; NCBI TaxID: 118562; scientific name: Tetraselmis chuii; NCBI TaxID: 63592;
ModificationList	monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-16 07:18:38	ID requested
⏵ 1	2025-07-09 05:22:08	announced

10.1007/S00449-025-03192-8;

submitter keyword: In silico analysis, Bioactive peptides, Peptidomics approach, Enzymatic hydrolysis,Microalgae

Jean-Louis Hilbert
contact affiliation	UMR Transfrontalière BioEcoAgro N°1158, Université Lille, F-59000 Lille, France.
contact email	jean-louis.hilbert@univ-lille.fr
lab head
Barbara Deracinois
contact affiliation	UMRT BioEcoAgro
contact email	barbara.deracinois@univ-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058907
     1. Label: PRIDE project
     2. Name: Proteomics and bioinformatics guided discovery of microalgal multifunctional peptides for novel nutraceutical applications