Salicylic acid, as a plant hormone, significantly affects physiological and biochemical indexes of such as soluble sugar, malondialdehyde content, peroxidase and superoxide dismutase enzyme activity in Platycodon grandiflorus. Lysine malonylation is a post-translational modification that involves various cellular functions in plants, though it is rarely studied, especially in medicinal plants. This study aimed to perform a comparative quantitative proteomic study of malonylation modification on P. grandiflorus root proteins after salicylic acid treatment using Western Blot with specific antibodies, affinity enrichment and LC-MS/MS analysis methods. The analysis identified 1,907 malonyl sites for 809 proteins, with 414 proteins and 798 modification sites quantified with high confidence. Post-treatment, 361 proteins were up-regulated, and 310 were down-regulated. Bioinformatics analysis revealed that malonylation in P. grandiflorus is primarily involved in pho-tosynthesis and carbon metabolism. Physiological and biochemical analysis showed that saliylic acid treatment increased the malondialdehyde levels, soluble protein, superoxide dismutase, and peroxidase activity but did not significantly affect the total saponins content in P. grandiflorus. These findings provide an important basis for exploring the molecular mechanisms of P. grandi-florus following salicylic acid treatment and enhance understanding of the biological function of protein lysine malonylation in plants.