PXD058480

PXD058480 is an original dataset announced via ProteomeXchange.

Title	Elongator is a microtubule polymerase selective for poly-glutamylated tubulin: ELP456 to Tubulin crosslinking
Description	Elongator is a tRNA-modifying complex regulating protein translation. Recently, a moonlighting function of Elongator has been identified in regulating the polarization of the microtubule cytoskeleton during asymmetric cell division. Elongator induces symmetry breaking of the anaphase midzone by selectively stabilizing microtubules on one side of the spindle, contributing to the downstream polarized segregation of cell-fate determinants and therefore to cell fate determination. Here, we show how Elongator controls microtubule dynamics. Elongator binds simultaneously to the tip of microtubules and also to free GTP-tubulin heterodimers using two different subcomplexes, Elp123 and Elp456, respectively. We show that these activities must be coupled for Elongator to decrease the critical concentration of tubulin for microtubule elongation. As a consequence, Elongator increases microtubule growth speed and decreases their catastrophe rate. Surprisingly, we found that Elp456 binds to tubulin tails and has strong selectivity towards polyglutamylated tubulin. Hence, microtubules assembled by Elongator become selectively enriched with polyglutamylated tubulin. Therefore, Elongator rewrites the tubulin code of growing microtubules, placing it at the core of cytoskeletal dynamics and polarization during asymmetric cell division.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:30:23.192.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomos Morgan
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-02 08:05:02	ID requested
⏵ 1	2025-05-07 05:30:24	announced

10.1038/s44318-024-00358-0;

submitter keyword: Tubulin Crosslinking ELP456

Emmanuel Derivery
contact affiliation	Laboratory of Molecular Biology
contact email	derivery@mrc-lmb.cam.ac.uk
lab head
Tomos Morgan
contact affiliation	MRC-LMB
contact email	tmorgan@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058480
     1. Label: PRIDE project
     2. Name: Elongator is a microtubule polymerase selective for poly-glutamylated tubulin: ELP456 to Tubulin crosslinking