This study aimed to describe the proteomic profile of Conus regius venom through proteomic analysis. Venom samples were fractionated using reversed-phase chromatography and analyzed by Q-TOF mass spectrometry. The resulting spectra were analyzed using Peaks Studio V7.0 software and a Conus protein database. A total of 520 peptide and protein sequences were identified, primarily in the 5-10 kDa molecular mass range, with most corresponding to conopeptides.