In our study, we collected urine samples from 246 cases, including 131 BC patients, 27 benign bladder disease patients, and 88 healthy adults as the control group. Ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) was applied for urine metabolomics analysis to distinguish the BC group from the healthy controls and the benign group, and the low-grade BC group from the high-grade BC group.