PXD058141

PXD058141 is an original dataset announced via ProteomeXchange.

Title	Roles of stress granule formation in cancer cell behaviors
Description	Although several studies have reported that overexpression of certain SG proteins can enhance cancer progression, the role of SGs, per se, in this process has not been clearly determined. To address this issue, we expressed wild-type (EGFP-G3BP1) or mutant G3BP1 (EGFP-G3BP1-ΔNTF2, which lacks the G3BP dimerization domain) in G3BP1/2 knockout (KO) cells. G3BP is the most critical core protein for stress granule formation. The cells were then treated with either sodium arsenite (100 μM for 1 hour) or FAK inhibitor (FAKi, FAK14 at 20 μM for 4 hours) to induce stress granules. An EGFP-empty sample was also included as the negative control for the proteomic analysis in the experiment. Each sample was pulled down by GFP-trap system to identify SG components under these specific stress conditions.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_08:06:42.565.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058141
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Seungwon Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-21 09:11:34	ID requested
⏵ 1	2025-09-17 08:06:42	announced

10.6019/PXD058141;

submitter keyword: Anchorage independence, Anoikis stress, Cancer progression,Stress granule

Pavel Ivanov
contact affiliation	Division of Rheumatology, Inflammation, and Immunity, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA
contact email	pivanov@bwh.harvard.edu
lab head
Seungwon Yang
contact affiliation	Brigham Women's Hospital (Harvard Medical School)
contact email	syang45@bwh.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD058141

PRIDE project URI

• PRIDE
  1. PXD058141
     1. Label: PRIDE project
     2. Name: Roles of stress granule formation in cancer cell behaviors