This project investigates the impact of different delivery modes and labor on maternal and neonatal health by analyzing 40 participants, including 16 spontaneous vaginal deliveries (VD), 16 prelabor cesarean deliveries (CS), and 8 intrapartum cesarean sections (Intra_CS). Using label-free proteomics and untargeted metabolomics, both amniotic fluid and cord blood samples were analyzed respectively to identify variations in protein and metabolite profiles associated with the mode of delivery. The study aims to uncover biological pathways influenced by labor and delivery mode, providing insights into how these factors shape maternal and neonatal outcomes, with implications for improving perinatal care and long-term health strategies.