The peach-potato aphid, Myzus persicae, is a globally pervasive crop pest, primarily reproducing clonally, allowing single females to rapidly generate exact copies of themselves. Moreover, its extreme polyphagy enables it to colonize over 400 plant species. Studies on the genetic underpinnings of M. persicae colonization have highlighted the differential expression and transcriptional regulation of a family of cysteine proteases, Cathepsin B (CathB), in response to various plant species. Among all M. persicae CathB proteins, CathB6 is most highly expressed in aphids on A. thaliana and most abundantly detected in M. persicae oral secretions. To investigate the potential plant target of CathB6 in A. thaliana, we conducted TurboID-based proximity labelling and MS (PL-MS).