LC-MSMS analysis of NVL2-associated pre-60S complex. To obtain more insights into the molecular mechanism of this effects caused by the mutant NVL2 in pre-60S complexes,we searched for additional proteins that preferentially interact with the NVL2(E365Q/E682Q) in comparison with wild-type NVL2. Co-mmunoprecipitation was performed by using FLAG-tagged NVL2 (wild-type or E365Q/E682Q) as baits and their interacting proteins were analyzed by LC-MS/MS.