To identify cGAS acetylation sites, 293T cells coexpressing the PCDH-EGFP-puro empty vector or PCDH-EGFP-puro-Flag-cGAS with KAT2A and MYO1F were subjected to immunoprecipitation with an anti-Flag antibody. After washing four times with IP buffer, the protein was eluted with IP buffer containing 0.1% SDS and analyzed by mass spectrometry.