The goal of our study was to experimentally quantify both at the transcriptomic and proteomic level the effects of comprehensive innate immune stimulations in chicken. To this end, SILAC-labeled chicken DF-1 cells were mock-treated or treated with either ChIFN1 (1000 IU/mL for 6h), transfected poly (I:C) (4,25 ug/mL for 6 h) or infected with Infectious Bursal Disease Virus strain PBG-98 (IBDV, MOI 5 for 8h). Total cellular RNA was extracted using RNeasy Mini Kit (QIAGEN) and Illumina libraries were prepared and sequenced. In parallel, proteins were extracted and processed for SILAC-based quantitative mass-spectrometry. Samples were run on a LTQ-Velos Orbitrap tandem mass spectrometer and the data processing and quantification were performed by the MaxQuant software.