PXD057537

PXD057537 is an original dataset announced via ProteomeXchange.

Title	Microbial metabolite Indole-3-propionic acid drives mitochondrial respiration in CD4+ T cells to confer protection against intestinal inflammation
Description	The gut microbiota and its metabolites critically regulate immune cell phenotype, function, and energy metabolism. We screened a collection of gut microbiota-related metabolites to identify modulators of mitochondrial metabolism in T cells. Here, we show that indole-3-propionic acid (IPA) stimulates mitochondrial respiration of CD4+ T cells by increasing the oxidation of fatty acids and amino acids (FAO and AAO), while inhibiting glycolytic capacity. IPA also impacts CD4+ T cell behavior by inhibiting their differentiation to TH1 and TH17 phenotypes. Mechanistically, the metabolic and immune effects of IPA are mediated by Peroxisome Proliferator-Activated Receptor-beta/delta. The administration of IPA rescues mitochondria respiration in mice with gut bacteria depletion or colitis by enhancing FAO and AAO in colonic CD4+ T cells. Adoptive transfer experiments show that IPA acts on CD4+T cells to exert its protective effect against inflammation. Collectively, our study reveals that the anti-inflammatory effects of IPA are mediated by metabolic reprogramming of CD4+ T cells toward the enhancement of mitochondrial respiration.
HostingRepository	PRIDE
AnnounceDate	2025-08-28
AnnouncementXML	Submission_2025-08-28_10:45:52.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-cysteine methyl disulfide; monohydroxylated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-05 06:49:07	ID requested
⏵ 1	2025-08-28 10:45:52	announced

Dataset with its publication pending

submitter keyword: Gut microbiota, Indole-3-propionic acid (IPA), Immunometabolism, Inflammatory bowel disease (IBD), CD4+ T cell

Harry Sokol
contact affiliation	Sorbonne Université, INSERM UMRS-938, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Paris, France.
contact email	harry.sokol@gmail.com
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057537
     1. Label: PRIDE project
     2. Name: Microbial metabolite Indole-3-propionic acid drives mitochondrial respiration in CD4+ T cells to confer protection against intestinal inflammation