Microalgae, particularly Chlamydomonas, produce valuable pigments like chlorophylls and carotenoids for industrial applications while demonstrating potential in bioremediation. This study employs proteomics to analyze protein expression patterns between norflurazon-resistant and non-resistant Chlamydomonas strains. The investigation aims to understand the molecular mechanisms underlying herbicide resistance, focusing on differential protein expression when exposed to norflurazon, a commonly used herbicide in weed control. This research could provide insights into both bioremediation capabilities and herbicide resistance mechanisms in microalgae.