The difficulty to obtain data from whom uninterruptedly practice meditation over many years, and lacking of advanced analysis methods significantly hinder the comprehensive understanding of molecular mechanisms underlying meditation. This study aimed to address this issue.Accordingly, we recruited 86 advanced Tibetan Buddhists and 59 matched Tibetan controls in Tibet . Coupled with multiple data independent acquisition and parallel reaction morning spectrometry techniques, we systematically investigated the molecular basis of how meditation affect cardiovascular protection.