The MDA-MB-231 cells were treated with CT-1 for 24 h. Then the cells were collected for proteomic analysis. SDT buffer was used for sample lysis and protein extraction. Protein digestion by trypsin was performed according to filter-aided sample preparation (FASP) procedure described by Matthias Mann. Then 100 μg peptide mixture of each sample was labeled using TMT reagent according to the manufacturer’s instructions. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer that was coupled to Easy nLC for 60/90 min. The MS raw data for each sample were searched using the MASCOT engine embedded into Proteome Discoverer 1.4 software for identification and quantitation analysis.