The semaphorins encompass a substantial family of secreted and transmembrane glycoproteins, originally recognized as pivotal factors in guiding axonal growth during the development of neural circuitry. Recent studies have uncovered diverse functions of semaphorins in a wide array of immune responses, encompassing immune cell activation, differentiation, migration, and cell-cell interactions, contingent upon their transmembrane receptors and co-receptors within specific biological contexts. However, a comprehensive investigation exploring the role, mechanism, and the interplay between SEMA3G and tumor immune surveillance remains elusive.