Dictyostelium discoideum O-Ser and O-Thr fucosylated proteins from WT, SPY-OE and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser and anti O-Thr fucose polyclonal antibodies in 3 Technical Replicates, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation), and SPY_OE (mutant, SPY overexpressor strain).