Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).