Harmful Algal Blooms involving the dinoflagellate Alexandrium pacificum continue to increase in marine ecosystems suffering the climate warming and anthropogenic pressure. Proteomics may contribute to better understand the responses of organisms exposed to environmental stress, thanks to the identification of stress proteins modified in their expression under such conditions. These proteins of interest can be enzymes involved in the regulation of metabolic pathways associated with adaptive proteomic response of an organism to face stresses. Present in mediterranean anthropized coastal ecosystems suffering the global warming, Alexandrium is a biological model of interest for studying the effects of global changes. To our knowledge and to date, no study has been carried out on the proteomic response of A. pacificum to global changes, such as warming and trace metal pollution. As a result, to enhance our understanding of this HAB species responses to the ongoing global changes, the present study aims at characterizing its proteomic and physiological (growth kinetics, morphometric measurements and PSTs) shifts under disturbed environmental conditions, i.e. using a mediterranean strain of A. pacificum successively exposed at 24°C, 27°C or 30°C, with or without polymetallic stress.