The nuclear basket (NB) as essential peripheral parts of nuclear pore complex (NPC), plays crucial roles in mRNA surveillance and transport, chromatin organization, and regulation of gene expression. However, the architectural and functional understanding of plant NB remains largely unknown. Using proximity labeling technology we identified a novel paralog of plant Nup50, designated Nup50c. Nup50c is predominantly located at the NB and interacts with plant-specific NB component Nup82. The C terminal region of Nup82 is responsible for the interaction with the canonical helixes of Nup50c and tethering it to the NPC.