To find the downstream signaling components upon CO4 or CO7 perception, we performed proteomics and phosphoproteomics analysis with M. paleacea treated with 10-5 M CO4 or CO7 for 10 mins. For the proteomics and phosphoproteomics analysis, 14-day-old gemmalings of M. paleacea growing on 1/2 Gamborg’s B5 medium (pH 5.8) containing 1% agar were sampled and floated on 2 ml sterilized water in a 6-well plate, and then incubated overnight. The water was replaced with the solution containing 10-5 M CO4, 10-5 M CO7, or mock for 10 min. The system consisted of a timsTOF Pro2 mass spectrometer (Bruker Daltonics) equipped with a nano elute system was used for proteomics and phosphoproteomics analysis.