Snake venoms have a very complex and chemically heterogeneous composition, in which the most abundant proteins and peptides play an important role in the immobilization and digestion of prey. It is obvious that the variability in the composition of the venom has a decisive influence on the designing of antivenoms. In this context, proteomic analyses are of great importance as they provide fundamental knowledge about the components of the venom. Therefore, the aim of the study was to investigate the proteomic profile of Vipera ammodytes ammodytes venom.