GST-TbCSBβC1 and GST proteins were expressed in Escherichia coli cells, and then incubated with total N.benthamiana proteins. The protein band that was specifically pulled-down by GST-TbCSB βC1 is indicated with a red arrow. GST is around 26 kDa and GST-IbCSB βC1 is around 40 kDa.