PXD056342

PXD056342 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based pipeline for screening hazardous proteins in alternative protein-based foods
Description	We present an effective non-targeted workflow for screening hazardous proteins in alternative protein-based foods (APBFs) that includes a protocol for efficient sample preparation, an optimised liquid chromatography with tandem mass spectrometry (LC-MS/MS) approach, and a data analytics pipeline with a comprehensive hazardous protein database. In addition, we developed a targeted MS assay for validating our screening results that confirmed the presence of some of selected hazardous proteins, and showed the quantification capability of this targeted assay at femtomole sensitivity.
HostingRepository	PRIDE
AnnounceDate	2025-07-07
AnnouncementXML	Submission_2025-07-07_09:17:08.216.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaowei Lou
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Glycine max; NCBI TaxID: 3847; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888; scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Fusarium venenatum; NCBI TaxID: 56646; scientific name: Vicia faba; NCBI TaxID: 3906; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-28 02:29:31	ID requested
⏵ 1	2025-07-07 09:17:08	announced

10.1016/J.FOODCHEM.2025.145395;

submitter keyword: Alternative protein-based food

Protein hazard screening

Non-targeted analysis

Targeted analysis

SureQuant

Jayantha Gunaratne
contact affiliation	Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Xiaowei Lou
contact affiliation	Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR)
contact email	lou_xiaowei@imcb.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD056342

PRIDE project URI

• PRIDE
  1. PXD056342
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based pipeline for screening hazardous proteins in alternative protein-based foods