PXD055952

PXD055952 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics informs discovery and delivery of Mycobacterium tuberculosis MHC-II antigens for vaccine design
Description	No currently licensed vaccine reliably prevents pulmonary tuberculosis, a leading cause of infectious disease mortality with a growing rate of extensive drug resistance. Developing effective new vaccines will likely require identifying Mycobacterium tuberculosis (Mtb)-derived antigens presented on MHC class II (MHC-II) by infected phagocytes that CD4+ T cells can recognize to mediate protective immunity. To prime T cells capable of recognizing infected cells, vaccines will also need to deliver antigens to appropriate processing pathways. Here, we use mass spectrometry (MS) to identify 27 Mtb-derived MHC-II peptides presented by infected human dendritic cells, derived from 13 source proteins. Using targeted quantitative MS, we assess and optimize the ability of vaccines to drive presentation of MHC-II epitopes that match those presented by Mtb-infected cells, including the existing Bacillus Calmette Guerin (BCG) vaccine and new mRNA-encoded immunogens. Our results identify potential antigenic targets and immunogen design principles for tuberculosis vaccine development.
HostingRepository	PRIDE
AnnounceDate	2025-09-18
AnnouncementXML	Submission_2025-09-17_18:14:48.463.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055952
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Owen Leddy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-17 13:36:10	ID requested
⏵ 1	2025-09-17 18:14:48	announced

10.6019/PXD055952;

10.1101/2024.10.02.616386;

submitter keyword: dendritic cells,tuberculosis,MHC class II,human,immunopeptidomics

Forest White
contact affiliation	Massachusetts Institute of Technology, Koch Institute for Integrative Cancer Research, Center for Precision Cancer Medicine
contact email	fwhite@mit.edu
lab head
Owen Leddy
contact affiliation	Massachusetts Institute of Technology
contact email	owenl@mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055952
     1. Label: PRIDE project
     2. Name: Immunopeptidomics informs discovery and delivery of Mycobacterium tuberculosis MHC-II antigens for vaccine design