The salivary gland plays a crucial role in oral function and overall health by secreting saliva, yet salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated salivary regenerative biobank at Mayo Clinic to study salivary gland stem/progenitor cells (SGSPCs). Optimization of cell isolation and progenitor assays revealed SGSPCs enriched within the CD24/EpCAM/CD49f+ and PSMA- phenotype of both submandibular and parotid glands, with clonal differentiation assays highlighting heterogeneity. Induction of PSMA/FOLH1 expression was associated with SGSPC differentiation. Utilizing label-free single-cell proteomics, we identified 819 unique proteins in SGSPC-enriched cells, including co-expressed cytokeratins, expressed in rare salivary ductal basal cells. Additionally, PRDX, a unique class of peroxiredoxin peroxidases enriched in SGSPCs demonstrated H2O2-dependent growth, suggesting a role in salivary homeostasis. These findings lay a solid foundation for studying SGSPCs and may lead to personalized regenerative medicine for salivary gland dysfunction.