We quantified the proteome of Dsc complex mutants (tul1gld1) and ESCRT mutants (vps4). Therefore we used stable isotope labeling with amino acids in cell culture (SILAC) to identify changes in the membrane proteome of tul1gld1orvps4 cells relative to WT cells or gld1 cells (labeled with “heavy” [13C6,15N2]-L-lysine).