Vriesea longistaminea is an epiphytic plant species found in the Brazilian Cerrado with ability to thrive in diverse soil conditions. This study presents a comparative proteomic analysis of V. longistaminea tissues collected from canga and quartzite soils, aiming to elucidate the molecular mechanisms underlying its adaptation to different soil substrates. Proteins were extracted from plant tissues and analyzed using LC-MS/MS. The results revealed significant differences in the proteomic profiles between V. longistaminea tissues from canga and quartzite soils. Proteins involved in stress response, nutrient uptake, and metabolism were found to be differentially expressed, suggesting that V. longistaminea adapts its proteome to cope with the distinct soil characteristics. Our findings provide valuable insights into the molecular basis of plant adaptation to edaphic factors and highlights the importance of proteomic analysis in understanding plant-soil interactions.