SUDHL4 cells were treated with vehicle control, 24uM gemcitabine alone, DMSO, 6uM AZD6738 alone, or 24uM Gemcitabine with 6uM AZD6738 for 6h, collected and subjected to CETSA heating, followed by cell lysis, isolation of soluble fraction and sample preparation for MS including digestion, desalting, TMT labeling, offline fractionation and pooling.