Venom protein fluid was solubilized in SDT lysis buffer (4% SDS, 100mM Tris-HCl, 1mM DTT, pH 7.6). The detergent, DTT and other low-molecular-weight components were removed by repeated ultrafiltration (Microcon units, 30 kD) using UA buffer (8 M Urea, 150 mM Tris-HCl pH 8.0). The protein suspensions were digested with trypsin (Promega) in 100mM NH4HCO3 buffer overnight at 37 °C, and the resulting peptides were collected as a filtrate. Peptide fragmentation using C18 Cartridge After the peptides were lyophilized, 0.1% formic acid solution was added to reconstitute, and the peptides were quantified (OD280). The peptide separation was performed using the Easy nLC HPLC liquid system with nanoliter flow rates. The peptides were separated by chromatography and analyzed by mass spectrometry using a Q-Exactive mass spectrometer (Thermo Fisher Scientific).