Updated project metadata. A blue-light receptor phototropin (phot) mediates chloroplast relocation responses. Upon receiving blue light, phot undergoes autophosphorylation via the serine/threonine kinase domain. The autophosphorylation levels of phot change in response to different blue-light intensities and temperature, and its level of autophosphorylation is associated with the induction of different types of chloroplast relocation responses. However, the phosphorylation sites of phot in the liverwort Marchantia polymorpha (Mpphot) is unknown. Here, we comprehensively surveyed serine and threonine residues as potential phosphorylation sites by using nano LC-MSMS and 23 phosphorylation sites and 18 candidates were identified.