The raw data of IP-MS analysis was analyzed in MaxQuant (version 2.0.1.0) software against the UniProt Mycolicibacterium smegmatis database (6602 sequences, Proteome ID: UP000000757). The trypsin/P was set as the protease and 2 missed cleavages were allowed. Carbamidomethyl (C) was set as the fixed modification, acetylation (Protein N-term) and oxidation (M) was set as variable modifications. The iBAQ values of each protein calculated through MaxQuant software were used for quantitation analysis. The quantitated proteins acquired in three of four biological replicates (fold-change higher than 2 or identified only in the experimental group) were defined as potential candidate proteins.