Deep vein thrombosis (DVT) is a leading cause of morbidity and mortality after trauma. Here, we integrate metabolomics and proteomics in up to 680 individuals with and without DVT after trauma (pt-DVT) to reveal the metabolic alterations and their function, and provide potential biomarkers. We carried out a nested case-control study design using a prospective cohort, which enrolled 680 Chinese Han subjects diagnosed with acute traumatic fractures at the Department of Trauma Surgery, Honghui Hospital (Xi’an, China) from October 2018 to October 2022. There were 183 subjects (DVT patients: N=96, Controls: N=87) were used for proteomics analysis.