PXD054008

PXD054008 is an original dataset announced via ProteomeXchange.

Title	Membrane proteomics in SHEP21N cells treated with NMTi
Description	N-myristoyltransferase inhibitors (NMTi) are promising novel anti-cancer agents, including in MYCN-amplified neuroblastoma and Burkitt’s lymphoma, but a mechanistic rationale for targeted therapy is still poorly defined. A key function of N-myristoylation is to mediate membrane localisation. We therefore carried out membrane proteomics on the SHEP21N cell line +/- NMTi. Identification of PPIs between affected proteins via STRING allowed the identification of heavily affected biological nodes, including respiratory complex I. This study provides a mechanistic rationale for NMTi in MYCN-amplified neuroblastoma.
HostingRepository	PRIDE
AnnounceDate	2025-07-27
AnnouncementXML	Submission_2025-07-27_13:44:49.016.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	myristoylated residue; acetylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-17 05:36:48	ID requested
⏵ 1	2025-07-27 13:44:49	announced

Dataset with its publication pending

submitter keyword: NMT, N-myristoylation, IMP-1088

Edward W.
contact affiliation	Department of Chemistry, Imperial College London, Molecular Research Science Hub, 82 Wood Lane, London W12 0BZ, United Kingdom. The Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom.
contact email	e.tate@imperial.ac.uk
lab head
James Zhang
contact affiliation	Impreial College London
contact email	james.zhang20@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD054008

PRIDE project URI

• PRIDE
  1. PXD054008
     1. Label: PRIDE project
     2. Name: Membrane proteomics in SHEP21N cells treated with NMTi