Activity-based protein profiling is a powerful tool to study target engagement in complex biological environments. XO44, ALX005 and ALX011 are chemical probes based on the scaffold of a promiscuous kinase binder, which covalently reacts with a conserved lysine in the ATP-binding pocket of kinases. Pretreatment of cells with a drug (candidate) results in competition of XO44/ALX005/ALX011 binding, which can be interpreted as in situ target engagement. Here CellEKT is presented, a workflow which is optimized for kinome coverage, reproducibility and data processing. This dataset pertains to the selectivity testing of Dasatinib in MV4-11 cells using a probe cocktail of XO44, ALX005 and ALX011.