PXD053900

PXD053900 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cysteine redoxome landscape in the liver of male mice fed a high-fat high sucrose diet
Description	Reversible cysteine post-translational modifications serve as a "switch" for protein structure-function dynamics. Herein, we established a strategy for comprehensively mapping the cysteine redoxome by pinpointing oxidized and reduced cysteine residues in the liver of male mice fed a high-fat/high-sucrose diet (HFHSD). We identified 3,151 and 2,361 labeled cysteine residues on 961 and 804 proteins in the normal chow diet and HFHSD groups, respectively. HFHSD shifts the cysteine redox states towards oxidation, especially in the mitochondria. The oxidized and reduced cysteine residues exclusively in the HFHSD group are enriched in cellular redox homeostasis, mitochondrial ATP metabolism, post-translational modifications, and disease-associated pathways. Exclusively reduced cysteine residues in the HFHSD group display a distinct consensus cysteine thiol motif, E[RK]C. The current cysteine redoxome strategy broadens the disease-associated proteome landscape and provides potential therapeutic target cysteine residues critical for regulating protein functions and interactions relevant to pathophysiology.
HostingRepository	jPOST
AnnounceDate	2025-07-12
AnnouncementXML	Submission_2025-07-11_08:00:04.589.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takumi Nishiuchi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-methionine sulfoxide; unknown modification; unknown modification; unknown modification; alpha-amino acetylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-11 20:26:48	ID requested
⏵ 1	2025-07-11 08:00:04	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: cysteine, post-translational modifications, proteomics, high-fat/high-sucrose diet

submitter keyword: cysteine, post-translational modifications, proteomics, high-fat/high-sucrose diet

Contact List

Toshinari Takamura
lab head
Takumi Nishiuchi
contact affiliation	Kanazawa University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003199/