PXD053750

PXD053750 is an original dataset announced via ProteomeXchange.

Title	An INS-1 𝛽-cell proteome highlights the role of fatty acid biosynthesis in glucose-stimulated insulin secretion
Description	Pancreatic beta cells secrete insulin as a response to rising glucose levels in the blood, a process that is known as glucose-stimulated insulin secretion (GSIS). Although GSIS has been extensively studied, not all associated mechanisms are fully understood at the molecular level. In this study, we used liquid chromatography tandem mass spectrometry and data-independent acquisition to acquire proteomes of rat pancreatic INS-1 832/13 beta cells that were short-term stimulated with glucose concentrations ranging from 0 to 20 mM, quantifying the behavior of 3703 proteins across 11 conditions. Insulin secretion and ensemble clustering of proteome profiles revealed unique response patterns of proteins expressed by INS-1 cells. 237 proteins, amongst them proteins associated with SNARE interaction in vesicular transport, protein export, and pancreatic secretion showed an increase in abundance upon glucose stimulation, whilst the majority of proteins, including those associated with common metabolic pathways such as glycolysis, the TCA cycle and the respiratory chain, did not respond to rising glucose concentrations. Interestingly, we observe that certain proteins, for example enzymes participating in fatty acid metabolism, respond distinctly, showing a “switch-on” response upon release of glucose starvation with no further changes in abundance upon increasing glucose levels. We speculate that increased activity of fatty acid metabolic activity might either be part of GSIS by replenishing membrane lipids required for vesicle mediated exocytosis, and/or by providing an electron sink to compensate for the increase in glucose catabolism. This submission contains the raw files, the library used for the analysis, and the corresponding DIA-NN report and associated files.
HostingRepository	PRIDE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-07_16:34:31.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Muenzner
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 04:28:56	ID requested
⏵ 1	2025-07-07 16:34:31	announced

Dataset with its publication pending

submitter keyword: beta cells, insulin secretion, glucose stimulation,quantitative proteomics

Markus Ralser
contact affiliation	Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany The Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7BN, UK Max Planck Institute for Molecular Genetics, Berlin, Germany
contact email	markus.ralser@charite.de
lab head
Julia Muenzner
contact affiliation	Charite University Medicine, Berlin, Germany
contact email	julia.muenzner@charite.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD053750

PRIDE project URI

• PRIDE
  1. PXD053750
     1. Label: PRIDE project
     2. Name: An INS-1 𝛽-cell proteome highlights the role of fatty acid biosynthesis in glucose-stimulated insulin secretion