PXD053704
PXD053704 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Reproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and reaction monitoring mass spectrometry with stable isotope-labelled internal standards |
| Description | When using a mix of 274 light synthetic peptide standards as surrogates for 270 human plasma proteins, as well as stable isotope-labelled standards as normalizers for targeted quantitative analysis by LC/MRM-MS, the Seer Proteograph allowed for the enrichment and absolute quantitation of up to an additional 44% of protein targets (median) as well as improved reproducibility compared to a traditional proteomic workflow with no fractionation (median 8.3% vs. 13.1% CV). As the Proteograph technology gains popularity, establishing Proteograph-specific protein reference ranges and comparisons to methods like ELISA and LC/MRM-MS may be explored to enable absolute quantification of plasma proteins based on Proteograph data. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2025-02-17 |
| AnnouncementXML | Submission_2025-02-17_14:21:19.735.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Claudia Gaither |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | 6495C Triple Quadrupole LC/MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-05 15:57:50 | ID requested | |
| ⏵ 1 | 2025-02-17 14:21:20 | announced |
Publication List
| Gaither C, Popp R, Gajadhar AS, Borchers CH, Reproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and multiple reaction monitoring mass spectrometry with stable isotope-labelled internal standards. Analyst, 150(2):353-361(2025) [pubmed] |
Keyword List
| submitter keyword: plasma proteomics, LC/MRM-MS, fractionation, absolute quantitation, Seer Proteograph |
Contact List
| Christoph Borchers | |
|---|---|
| contact affiliation | McGill University |
| contact email | christoph.borchers@mcgill.ca |
| lab head | |
| Claudia Gaither | |
| contact affiliation | MRM Proteomics Inc |
| contact email | cgaither@mrmproteomics.com |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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